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KMID : 0381919990290040499
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Korean Journal of Microscopy
1999 Volume.29 No. 4 p.499 ~ p.509
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Terminal Dilation and Transformation of the Protein-filled ER to Form Protein Bodies in Pea (Pisum sativum L. var, exzellenz) Cotyledons
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Jeong Byung-Kap
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Abstract
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Accumulations of the storage proteins in protein storage vacuole and the differentiation of protein bodies from protein-filled ER in developing pea cotyledons have been investigated using conventional and immunoelectron microscopy. To improve the fixation quality, single cells separated enzymatically from sliced cotyledons were used. At early stages of seed development osmiophilic protein accumulates in rER lumen were observed quite often. This protein-filled ER cisternae were differentiated into cytoplasmic protein bodies at late stage by the process called terminal dilations which have been considered a principal route of the formation of cytoplasmic protein bodies somewhat later in seed maturation. Immunocytochemical labellings of the vicilin and legumin show that presence of vicilin on both of the cytoplasmic PB and PD, but limited presence of legumin only on the cytoplasmic PB at intermediate stage of seed development. Immunogold labellings of Bip, ER retention protein, were observed on the inner periphery of protein deposits in protein storage vacuole. This result was regarded that Bip can recognize and retrieve misfolded protein during active accumulation of storage protein to the PD in PSV.
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KEYWORD
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Pea, Storage protein, Protein-filled ER, Dilation, Immunogold labellings
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